Trypanosomiasis is an infectious emerging hemoprotozoan parasitic disease in domestical animals of Nepal. The prevalence of disease was found in 16 of 240 (6.67%) in domestic animals of Makawanpur district, out of which 9 of 105 were (8.57%) cattle; 5 of 75 (6.67%) buffalos, and 2 of 15 (13.3%) dogs, while none of the goats and pigs acquired infection. The disease was found maximum during rainy season 9 of 82 (10.98%) with higher prevalence among cross breeds than that of local breeds.