Abstract
The bacterial endosymbiont Wolbachia of several species of filarial nematodes plays an important role in the inflammatory pathology of filariasis. Nitric oxide (NO) production has also been implicated in the immune response during filarial infections. Here we present data indicating that a recombinant Wolbachia surface protein (rWSP) induces iNOs mRNA expression and NO production, as well as IFN-gamma and a Th1-type antibody response, in inoculated BALB/c mice. This effect is not observed when mice are inoculated with a recombinant heat shock protein from Wolbachia (GroEL).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Bacterial / biosynthesis
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Bacterial Outer Membrane Proteins / immunology*
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Chaperonin 60 / immunology
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Dirofilaria immitis / microbiology*
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Dirofilaria immitis / physiology
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Dirofilariasis / immunology
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Dirofilariasis / parasitology
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Inflammation / immunology
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Interferon-gamma / genetics
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Interferon-gamma / immunology
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Interleukin-10 / genetics
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Interleukin-4 / genetics
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Mice
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Mice, Inbred BALB C
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / genetics
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Nitric Oxide Synthase Type II / metabolism*
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RNA, Messenger
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Recombinant Proteins / immunology
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Symbiosis
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Th1 Cells / immunology
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Wolbachia / immunology*
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Wolbachia / physiology
Substances
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Antibodies, Bacterial
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Bacterial Outer Membrane Proteins
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Chaperonin 60
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RNA, Messenger
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Recombinant Proteins
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Wsp protein, Wolbachia
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Interleukin-10
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Interleukin-4
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Nitric Oxide
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Interferon-gamma
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse