Does fluorescence of ANS reflect its binding to PAMAM dendrimer?

D Shcharbin; M Szwedzka; M Bryszewska

doi:10.1016/j.bioorg.2006.10.003

Does fluorescence of ANS reflect its binding to PAMAM dendrimer?

Bioorg Chem. 2007 Apr;35(2):170-4. doi: 10.1016/j.bioorg.2006.10.003. Epub 2006 Nov 28.

Authors

D Shcharbin¹, M Szwedzka, M Bryszewska

Affiliation

¹ Department of General Biophysics, University of Lodz, 12/16 Banacha Street, 90-237 Lodz, Poland.

PMID: 17126376
DOI: 10.1016/j.bioorg.2006.10.003

Abstract

The analysis of binding between cationic PAMAM G5 dendrimer and anionic fluorescent probe using fluorescence and equilibrium dialysis has been made. It was found that at low concentrations of ANS the double fluorimetric titration technique can be successfully used for quantitative analysis of binding of ANS to dendrimer. Based on fluorescence and dialysis data the constants of binding and the number of binding centers were calculated for binding of ANS to PAMAM G5 dendrimer: K(b) is approx. (0.5-1)x10(5)M(-1) and n is (0.5-0.7).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Anilino Naphthalenesulfonates / chemistry*
Dendrimers
Dialysis
Fluorescence
Fluorescent Dyes / chemistry*
Polyamines / chemistry*
Spectrometry, Fluorescence

Substances

Anilino Naphthalenesulfonates
Dendrimers
Fluorescent Dyes
PAMAM Starburst
Polyamines
1-anilino-8-naphthalenesulfonate