Abstract
Two primer sets for automated ribosomal intergenic spacer analysis (ARISA) were used to assess the bacterial community composition (BCC) in Lake Mendota, Wisconsin, over 3 years. Correspondence analysis revealed differences in community profiles generated by different primer sets, but overall ecological patterns were conserved in each case. ARISA is a powerful tool for evaluating BCC change through space and time, regardless of the specific primer set used.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Automation
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Bacteria / classification*
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Bacteria / genetics
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Bacteria / growth & development
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Bacteria / isolation & purification
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DNA Primers*
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DNA, Bacterial / analysis
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DNA, Ribosomal Spacer / analysis*
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Ecosystem*
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Fresh Water / microbiology*
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Multivariate Analysis
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RNA, Ribosomal, 16S / genetics
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RNA, Ribosomal, 23S / genetics
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Seasons
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Wisconsin
Substances
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DNA Primers
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DNA, Bacterial
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DNA, Ribosomal Spacer
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RNA, Ribosomal, 16S
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RNA, Ribosomal, 23S