Comparison of primer sets for use in automated ribosomal intergenic spacer analysis of aquatic bacterial communities: an ecological perspective

Appl Environ Microbiol. 2007 Jan;73(2):659-62. doi: 10.1128/AEM.02130-06. Epub 2006 Nov 22.

Abstract

Two primer sets for automated ribosomal intergenic spacer analysis (ARISA) were used to assess the bacterial community composition (BCC) in Lake Mendota, Wisconsin, over 3 years. Correspondence analysis revealed differences in community profiles generated by different primer sets, but overall ecological patterns were conserved in each case. ARISA is a powerful tool for evaluating BCC change through space and time, regardless of the specific primer set used.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Automation
  • Bacteria / classification*
  • Bacteria / genetics
  • Bacteria / growth & development
  • Bacteria / isolation & purification
  • DNA Primers*
  • DNA, Bacterial / analysis
  • DNA, Ribosomal Spacer / analysis*
  • Ecosystem*
  • Fresh Water / microbiology*
  • Multivariate Analysis
  • RNA, Ribosomal, 16S / genetics
  • RNA, Ribosomal, 23S / genetics
  • Seasons
  • Wisconsin

Substances

  • DNA Primers
  • DNA, Bacterial
  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S