Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts

FEBS Lett. 2006 Nov 27;580(27):6455-63. doi: 10.1016/j.febslet.2006.10.064. Epub 2006 Nov 7.

Abstract

Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38(MAPK) was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38(MAPK) activity attenuated the increase in the proportion of cells positive for senescence associated beta-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38(MAPK) inhibitor and p38(MAPK) small interferent RNA, we identified several p38(MAPK)-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cellular Senescence / drug effects*
  • Cellular Senescence / genetics
  • Fibroblasts / enzymology*
  • Gene Expression Profiling / methods
  • Gene Expression Regulation / drug effects*
  • Gene Expression Regulation / genetics
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • Oligonucleotide Array Sequence Analysis / methods
  • Oxidants / pharmacology*
  • Protein Kinase Inhibitors / pharmacology
  • RNA, Small Interfering / genetics
  • Telomerase / biosynthesis*
  • Telomerase / genetics
  • p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Oxidants
  • Protein Kinase Inhibitors
  • RNA, Small Interfering
  • Hydrogen Peroxide
  • p38 Mitogen-Activated Protein Kinases
  • Telomerase