Abstract
Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38(MAPK) was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38(MAPK) activity attenuated the increase in the proportion of cells positive for senescence associated beta-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38(MAPK) inhibitor and p38(MAPK) small interferent RNA, we identified several p38(MAPK)-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Cellular Senescence / drug effects*
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Cellular Senescence / genetics
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Fibroblasts / enzymology*
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Gene Expression Profiling / methods
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Gene Expression Regulation / drug effects*
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Gene Expression Regulation / genetics
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Humans
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Hydrogen Peroxide / pharmacology*
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Oligonucleotide Array Sequence Analysis / methods
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Oxidants / pharmacology*
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Protein Kinase Inhibitors / pharmacology
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RNA, Small Interfering / genetics
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Telomerase / biosynthesis*
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Telomerase / genetics
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p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors
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p38 Mitogen-Activated Protein Kinases / metabolism
Substances
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Oxidants
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Protein Kinase Inhibitors
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RNA, Small Interfering
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Hydrogen Peroxide
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p38 Mitogen-Activated Protein Kinases
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Telomerase