Abstract
The effects of docosahexaenoic acid (DHA) and other fatty acids on P2X-receptor-mediated inward currents in rat nodose ganglion neurons were studied using the nystatin perforated patch-clamp technique. DHA accelerated the desensitization rate of the ATP-induced current. DHA showed use-dependent inhibition of the peak ATP-induced current. Other polyunsaturated fatty acids, such as arachidonic acid and eicosapentaenoic acid, displayed a similar use-dependent inhibition. The inhibitory effects of saturated fatty acids including palmitic acid and arachidic acid were weaker than those of polyunsaturated fatty acids. The results suggest that fatty acids may modulate the P2X receptor-mediated response when the channel is in the open-state.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / antagonists & inhibitors*
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Adenosine Triphosphate / pharmacology*
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Animals
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Data Interpretation, Statistical
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Docosahexaenoic Acids / pharmacology*
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Dose-Response Relationship, Drug
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Electrophysiology
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Fatty Acids / pharmacology*
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Ion Channels / drug effects
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Ion Channels / metabolism
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Membrane Potentials / drug effects
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Neurons / drug effects
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Neurons / metabolism*
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Nodose Ganglion / cytology
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Nodose Ganglion / drug effects
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Nodose Ganglion / metabolism*
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Patch-Clamp Techniques
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Rats
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Rats, Wistar
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Receptors, Purinergic P2 / drug effects
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Receptors, Purinergic P2 / physiology
Substances
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Fatty Acids
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Ion Channels
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Receptors, Purinergic P2
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Docosahexaenoic Acids
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Adenosine Triphosphate