Capillary electrophoresis fingerprinting, quantification and mass-identification of various 9-aminopyrene-1,4,6-trisulfonate-derivatized oligomers derived from plant polysaccharides

J Chromatogr A. 2006 Dec 22;1137(1):119-26. doi: 10.1016/j.chroma.2006.10.058. Epub 2006 Nov 7.

Abstract

Various plant polysaccharide derived mono- and oligosaccharides were derivatized with the fluorescent 9-aminopyrene-1,4,6-trisulfonate (APTS) and subjected to capillary electrophoresis (CE) in combination with laser induced fluorescence (LIF) detection. CE-LIF was suitable for mol-based quantification of various APTS-monosaccharides. CE-LIF of APTS-oligosaccharides showed high resolutions, while analysis times were at maximum 15 min. The coupling of CE to electrospray-iontrap mass spectrometery (MS) with online UV detection showed to be a powerful technique in the identification of APTS-oligosaccharides. For the first time, various APTS-xylo-oligosaccharides, having either no, O-acetyl, arabinosyl or xylosyl substitutions at varying positions, were identified by using CE-LIF and CE-MS(n).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabinose / analogs & derivatives
  • Chromatography, High Pressure Liquid
  • Electrophoresis, Capillary / methods*
  • Fluorescence
  • Lasers
  • Mass Spectrometry / methods*
  • Oligosaccharides / analysis
  • Oligosaccharides / isolation & purification
  • Plants / chemistry
  • Polysaccharides / analysis*
  • Pyrenes / analysis*
  • Pyrenes / isolation & purification
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sulfonic Acids / analysis*
  • Sulfonic Acids / isolation & purification
  • Ultraviolet Rays
  • Xylose / analogs & derivatives

Substances

  • 9-aminopyrene-1,4,6-trisulfonic acid
  • Oligosaccharides
  • Polysaccharides
  • Pyrenes
  • Sulfonic Acids
  • Xylose
  • Arabinose