Abstract
Carbon tetrachloride (CCl(4)) causes chronic hepatitis, featuring an increase in hepatic hydroxyproline, spleen weight and serum GPT levels and a decrease in plasma albumin levels. Crude extracts of fresh whole plants of Anoectochilus formosanus showed inhibition of chronic hepatitis induced by CCl(4) in mice. Bioactivity-guided fractionation and spectroscopic analysis revealed that kinsenoside was the most active compound. In an in vitro study, the LD(50) values for H(2)O(2)-induced cytotoxicity in BALB/c normal liver cells were significantly higher after kinsenoside pretreatment than after vehicle alone, further confirming that kinsenoside shows significant antihepatotoxic activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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4-Butyrolactone / analogs & derivatives
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Animals
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Carbon Tetrachloride
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Chemical and Drug Induced Liver Injury / blood
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Chemical and Drug Induced Liver Injury / diagnosis
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Chemical and Drug Induced Liver Injury / prevention & control*
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Lethal Dose 50
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Male
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Mice
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Monosaccharides / administration & dosage
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Monosaccharides / chemistry
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Monosaccharides / pharmacology*
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Monosaccharides / therapeutic use
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Orchidaceae*
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Phytotherapy*
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Plant Extracts / administration & dosage
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Plant Extracts / chemistry
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Plant Extracts / pharmacology
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Plant Extracts / therapeutic use
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Protective Agents / administration & dosage
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Protective Agents / pharmacology*
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Protective Agents / therapeutic use
Substances
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3-glucopyranosyloxybutanolide
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Monosaccharides
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Plant Extracts
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Protective Agents
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Carbon Tetrachloride
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4-Butyrolactone