Abstract
Covalent labeling has been widely used for structural and functional analyses of proteins. To target a wide range of PDZ domains, we designed a chemical scaffold mimicking the E/D-T/S-XV peptide, which is a PDZ domain that binds ligands in higher occurrence. A chemical probe (2) that contained this moiety alkylated diverse PDZ domains, including NHERF-1 PDZ2, and differentially visualized the cellular proteome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkylation
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Carrier Proteins / drug effects*
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Drug Design
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Electrophoresis, Polyacrylamide Gel
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Indicators and Reagents
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Ligands
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Membrane Proteins
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Molecular Probes / chemical synthesis*
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Molecular Probes / pharmacology*
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Peptides / chemical synthesis
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Peptides / pharmacology
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Phosphoproteins / chemistry
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Protein Binding
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Proteome / drug effects
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Sodium-Hydrogen Exchangers / chemistry
Substances
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Carrier Proteins
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Indicators and Reagents
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Ligands
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Membrane Proteins
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Molecular Probes
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PDZK1 protein, human
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Peptides
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Phosphoproteins
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Proteome
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Sodium-Hydrogen Exchangers
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sodium-hydrogen exchanger regulatory factor