High concentration calcium carbonate in calcareous soils usually limits plant Zn uptake. In this paper, a solution culture experiment was conducted to study the effects of CaCO3 addition with or no Zn supply on the growth and Zn uptake of three genotypes winter wheat seedlings. The results showed that Zn supply or CaCO3 addition had no significant effects on seedlings biomass and root/canopy ratio, and there was no significant difference among the three genotypes. CaCO3 addition led to leaf chlorosis. No matter with or no Zn supply, CaCO3 addition had no significant effects on the Zn concentration and accumulation in wheat root, stem, and leaf. Zn supply increased the Zn concentration in Yuanfeng 998, Zhongyu 6, and Xiaoyan 22 by 80.0%, 104.8% and 139.6%, respectively. For Yuanfeng 998, a sensitive genotype to Zn-deficiency, the increment of Zn concentration and accumulation was much lower than that of Zhongyu 6 and Xiaoyan 22, non-sensitive genotypes to Zn-deficiency. Zn supply or CaCO3 addition had no significant effects on the P content in root, stem, and leaf, but the corresponding P content of Yuanfeng 998 was obviously lower than that of the other two genotypes. Zn supply greatly decreased the P/Zn ratio in root, stem and leaf, and CaCO3 addition also showed the same tendency. Under Zn-deficiency, CaCO3 addition caused leaf chlorosis, but did not decrease wheat plant Zn uptake. In conclusion, at least under solution culture condition, high concentration calcium carbonate had no obvious inhibitory effect on plant zinc uptake, and whether this situation was true in calcareous soils is worthy to be further investigated.