Abstract
Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 Kd. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Protozoan / immunology
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Cell Separation
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Clone Cells
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Disease Models, Animal
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Epitopes / immunology
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Immunotherapy, Adoptive
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Malaria / immunology*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Peptide Fragments / chemical synthesis
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Peptide Fragments / immunology
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Plasmodium berghei / immunology
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Plasmodium yoelii / immunology*
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Protozoan Proteins*
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Spleen / cytology
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T-Lymphocytes, Cytotoxic / immunology*
Substances
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Antigens, Protozoan
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Epitopes
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Peptide Fragments
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Protozoan Proteins
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circumsporozoite protein, Protozoan