Isolation and characterization of protective cytolytic T cells in a rodent malaria model system

Immunol Lett. 1990 Aug;25(1-3):27-31. doi: 10.1016/0165-2478(90)90086-6.

Abstract

Protective immunity against malaria is induced by immunization with irradiation-attenuated sporozoites. Here we report the isolation of cytolytic T-cell (CTL) clones from BALB/c (H-2d) mice immunized with either Plasmodium berghei or Plasmodium yoelii sporozoites. The epitopes recognized by these CTL can be mimicked by synthetic peptides corresponding to a homologous region in the CS proteins of both rodent malaria species. Both peptides are recognized by the CTL in the context of the same MHC class I molecule, H-2 Kd. In vivo adoptive transfer of the CTL clones into non-immune syngeneic mice protected them from a lethal challenge of infectious sporozoites.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Protozoan / immunology
  • Cell Separation
  • Clone Cells
  • Disease Models, Animal
  • Epitopes / immunology
  • Immunotherapy, Adoptive
  • Malaria / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / immunology
  • Plasmodium berghei / immunology
  • Plasmodium yoelii / immunology*
  • Protozoan Proteins*
  • Spleen / cytology
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Antigens, Protozoan
  • Epitopes
  • Peptide Fragments
  • Protozoan Proteins
  • circumsporozoite protein, Protozoan