Enantiomeric separation of underivatized small amines in conventional and on-chip capillary electrophoresis with contactless conductivity detection

Xiao Yang Gong; Peter C Hauser

doi:10.1002/elps.200600258

Enantiomeric separation of underivatized small amines in conventional and on-chip capillary electrophoresis with contactless conductivity detection

Electrophoresis. 2006 Nov;27(21):4375-82. doi: 10.1002/elps.200600258.

Authors

Xiao Yang Gong¹, Peter C Hauser

Affiliation

¹ Department of Chemistry, University of Basel, Basel, Switzerland.

PMID: 17024687
DOI: 10.1002/elps.200600258

Abstract

The determination of the enantiomers of small non-UV-absorbing amines which otherwise can only be achieved with difficulty was possible by using a combination of the chiral crown ether (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18C6H4) and dimethyl-beta-CD as selectors in CE and contactless conductivity measurement for detection. Alkylamines without any other functional group, amino alcohols, species with ether or ester groups and with a cyclic moiety were investigated. The detection limits were found to be about 1.0 microM and the determination is possible up to at least 1.0 mM. The determination of enantiomeric ratios of up to 99.5:0.5 was also found feasible.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amines / chemistry
Amines / isolation & purification*
Electric Conductivity
Electrophoresis, Capillary / methods*
Electrophoresis, Microchip / methods*
Molecular Structure
Stereoisomerism

Substances

Amines