Abstract
We found that androgen and IGF-I up-regulated IGFBP-5 mRNA in androgen-responsive normal human fibroblast, which was blocked by co-treatment with 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole, but not cycloheximide. IGFBP-5 promoter activity was stimulated by androgen. Nuclear run-on assay revealed that IGFBP-5 transcripts were increased in response to androgen, which was not enhanced by co-addition of IGF-I. These results collectively indicate that IGFBP-5 synthesis is regulated by androgen through transcriptional mechanisms in androgen responsive cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cells, Cultured
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Fibroblasts / drug effects*
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Fibroblasts / metabolism
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Half-Life
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Humans
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Insulin-Like Growth Factor Binding Protein 5 / biosynthesis*
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Insulin-Like Growth Factor Binding Protein 5 / genetics
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Insulin-Like Growth Factor I / pharmacology
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Promoter Regions, Genetic
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RNA, Messenger / metabolism
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Receptors, Androgen / metabolism*
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Testosterone / pharmacology*
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Transcription, Genetic / drug effects*
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Transcriptional Activation
Substances
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Insulin-Like Growth Factor Binding Protein 5
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RNA, Messenger
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Receptors, Androgen
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Testosterone
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Insulin-Like Growth Factor I