IGF binding protein-5 synthesis is regulated by testosterone through transcriptional mechanisms in androgen responsive cells

Endocr J. 2006 Dec;53(6):811-8. doi: 10.1507/endocrj.k06-017. Epub 2006 Sep 26.

Abstract

We found that androgen and IGF-I up-regulated IGFBP-5 mRNA in androgen-responsive normal human fibroblast, which was blocked by co-treatment with 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole, but not cycloheximide. IGFBP-5 promoter activity was stimulated by androgen. Nuclear run-on assay revealed that IGFBP-5 transcripts were increased in response to androgen, which was not enhanced by co-addition of IGF-I. These results collectively indicate that IGFBP-5 synthesis is regulated by androgen through transcriptional mechanisms in androgen responsive cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Fibroblasts / drug effects*
  • Fibroblasts / metabolism
  • Half-Life
  • Humans
  • Insulin-Like Growth Factor Binding Protein 5 / biosynthesis*
  • Insulin-Like Growth Factor Binding Protein 5 / genetics
  • Insulin-Like Growth Factor I / pharmacology
  • Promoter Regions, Genetic
  • RNA, Messenger / metabolism
  • Receptors, Androgen / metabolism*
  • Testosterone / pharmacology*
  • Transcription, Genetic / drug effects*
  • Transcriptional Activation

Substances

  • Insulin-Like Growth Factor Binding Protein 5
  • RNA, Messenger
  • Receptors, Androgen
  • Testosterone
  • Insulin-Like Growth Factor I