The influence of temperature change on the determined concentrations of dissolved organic matter (DOM) in water was investigated by laser induced fluorescence (LIF) technique in laboratory. The temperature was altered within the range of 20 to 75 degrees C, and the intensity of DOM fluorescence and water Raman scattering were found to decrease with rising temperature. A linear fit of the normalized fluorescence intensity versus temperature yielded a mean value of the temperature coefficient of -5.24 x 10(-4) x degrees C(-1), and a mean value of the temperature coefficient of -3.42 x 10(-3)(mg x L(-1)) x degrees C(-1) was obtained according to the relationship between normalized fluorescence intensity and concentration of DOM. The temperature change will cause relative changes of up to 8.45% in DOM concentration within the range of 20 to 75 degrees C if we assume that the normalized fluorescence intensity remains the same.