Statistical properties of the band-broadening function

J Chromatogr A. 2006 Nov 10;1133(1-2):95-103. doi: 10.1016/j.chroma.2006.08.002. Epub 2006 Sep 1.

Abstract

Statistical properties of the band-broadening function calculated from the equilibrium and kinetic theories of Giddings and Eyring are compared with real liquid-chromatography elution curves of low-molecular-weight substances and with light-scattering elution curves of a high-molecular-weight polystyrene standard injected at several concentrations. The curves obtained by liquid chromatography agree in a large span of elution times with the theoretical ones and can be described by one value of the plate-height. The size exclusion chromatography (SEC) elution curves seem to approach the predicted statistical properties with decreasing concentration of injected polymer but, in general, a reliable prediction of band-broadening (BB) functions in SEC of polymers, especially of non-symmetric BB functions near the exclusion limit, seems not possible at this point. The relation of the theory with the characteristic-function approach developed by Dondi is also discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Gel / statistics & numerical data
  • Chromatography, Liquid / statistics & numerical data*
  • Models, Statistical
  • Scattering, Radiation