The purpose of this review is to compile and bring to the attention of the pediatric nephrology community various aspects of laboratory medicine pertinent to nephrology. The review addresses different aspects in laboratory medicine that should be taken into account during interpretation of a test result, such as methodological and analytical issues, statistical considerations and the biological interpretation of a test result in the context of the clinical setting. An understanding of the considerations and limitations in laboratory medicine will be helpful to the pediatric nephrologist when ordering and interpreting biochemical tests.