Toxicity in mice expressing short hairpin RNAs gives new insight into RNAi

Genome Biol. 2006;7(8):231. doi: 10.1186/gb-2006-7-8-231.

Abstract

Short hairpin RNAs can provide stable gene silencing via RNA interference. Recent studies have shown toxicity in vivo that appears to be related to saturation of the endogenous microRNA pathway. Will these findings limit the therapeutic use of such hairpins?

Publication types

  • Review

MeSH terms

  • Animals
  • Gene Silencing*
  • Genetic Therapy / adverse effects*
  • Genetic Vectors / toxicity
  • Immunity
  • Mice
  • MicroRNAs / metabolism
  • MicroRNAs / therapeutic use
  • MicroRNAs / toxicity
  • RNA Interference*
  • RNA Processing, Post-Transcriptional
  • RNA, Small Interfering / metabolism
  • RNA, Small Interfering / therapeutic use
  • RNA, Small Interfering / toxicity*

Substances

  • MicroRNAs
  • RNA, Small Interfering