To select appropriate plant materials for a phylogeography of deciduous broad-leaved forests in Japan, we surveyed intraspecific chloroplast DNA variation in 34 species found in these forests. A relatively large number of intraspecific cpDNA variations were detected in ten species: Carpinus japonica (nucleotide diversity pi=0.00083), C. laxiflora (pi=0.00221), Magnolia obovata (pi=0.00134), Lindera triloba (pi=0.00255), L. obtusiloba (pi=0.00289), Pourthiaea villosa var. leavis (pi=0.00263), Acer japonicum (pi=0.00170), A. micranthum (pi=0.00237), Euonymus oxyphyllus (pi=0.00322) and Styrax obassia (pi=0.00100).