Frozen tissue biobanks. Tissue handling, cryopreservation, extraction, and use for proteomic analysis

Acta Oncol. 2006;45(6):643-61. doi: 10.1080/02841860600818047.

Abstract

The potential and the limitations of protein analysis of tissue samples are surveyed. The complexity, concentration range and dynamics of the human proteome are reviewed, as is the effect of handling and cryopreservation. Protein extraction, solubilization, resolution and detection are discussed, in relation to the properties of the human proteome.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Cryopreservation*
  • Genetic Techniques
  • Humans
  • Proteins / isolation & purification*
  • Proteins / metabolism*
  • Proteome*
  • Proteomics / methods*
  • Specimen Handling / methods*
  • Tissue Banks*

Substances

  • Proteins
  • Proteome