Molecular characterisation of an Australian isolate of Bonamia exitiosa

Dis Aquat Organ. 2006 Jul 11;71(1):81-5. doi: 10.3354/dao071081.

Abstract

An Australian (New South Wales) isolate of Bonamia was characterised at the molecular level by sequencing the 18S-ITS-1 region of the small subunit rRNA operon obtained from flat oysters Ostrea angasi shown to be infected by histological examination. Sequence data alignment with homologous genes from 2 other isolates of Bonamia (New Zealand and France) revealed high levels of nucleotide identity with both isolates. However, the Australian Bonamia is shown to be more closely related to the New Zealand isolate, suggesting the existence of an oceanic subgroup of Bonamia.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers / chemistry
  • DNA, Protozoan / chemistry
  • DNA, Protozoan / isolation & purification
  • DNA, Ribosomal Spacer / genetics*
  • Haplosporida / genetics*
  • Haplosporida / isolation & purification
  • Molecular Sequence Data
  • New South Wales
  • Ostrea / parasitology*
  • RNA, Ribosomal, 18S / genetics*
  • Sequence Alignment

Substances

  • DNA Primers
  • DNA, Protozoan
  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 18S

Associated data

  • GENBANK/DQ312295