Serine/threonine protein kinase SpkA in Synechocystis sp. strain PCC 6803 is a regulator of expression of three putative pilA operons, formation of thick pili, and cell motility

Vladimir B Panichkin; Satoko Arakawa-Kobayashi; Toku Kanaseki; Iwane Suzuki; Dmitry A Los; Sergey V Shestakov; Norio Murata

doi:10.1128/JB.00838-06

Serine/threonine protein kinase SpkA in Synechocystis sp. strain PCC 6803 is a regulator of expression of three putative pilA operons, formation of thick pili, and cell motility

J Bacteriol. 2006 Nov;188(21):7696-9. doi: 10.1128/JB.00838-06. Epub 2006 Aug 17.

Authors

Vladimir B Panichkin¹, Satoko Arakawa-Kobayashi, Toku Kanaseki, Iwane Suzuki, Dmitry A Los, Sergey V Shestakov, Norio Murata

Affiliation

¹ National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan.

Abstract

Previous studies showed that a Ser/Thr protein kinase, SpkA, in Synechocystis sp. strain PCC 6803 is involved in cell motility. The present study, in which DNA microarray analysis and electron microscopy were used, demonstrated that SpkA regulates the expression of putative pilA9-pilA10-pilA11-slr2018, pilA5-pilA6, and pilA1-pilA2 operons and is essential for the formation of thick pili.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / physiology*
Fimbriae, Bacterial / genetics*
Fimbriae, Bacterial / ultrastructure
Gene Expression Profiling
Gene Expression Regulation, Bacterial*
Microscopy, Electron, Transmission
Movement
Oligonucleotide Array Sequence Analysis
Operon*
Protein Kinases / physiology*
RNA, Bacterial / analysis
RNA, Messenger / analysis
Synechocystis / genetics*
Synechocystis / physiology*
Synechocystis / ultrastructure
Transcription, Genetic

Substances

Bacterial Proteins
RNA, Bacterial
RNA, Messenger
Protein Kinases
spkA protein, Synechocystis