32P- and biotin-labelled in vitro transcribed cRNA probes for the detection of potato spindle tuber viroid and chrysanthemum stunt viroid

T Candresse; G Macquaire; V Brault; M Monsion; J Dunez

doi:10.1016/0923-2516(90)90059-r

32P- and biotin-labelled in vitro transcribed cRNA probes for the detection of potato spindle tuber viroid and chrysanthemum stunt viroid

Res Virol. 1990 Jan-Feb;141(1):97-107. doi: 10.1016/0923-2516(90)90059-r.

Authors

T Candresse¹, G Macquaire, V Brault, M Monsion, J Dunez

Affiliation

¹ INRA, Centre de Recherches de Bordeaux, Pont de la Maye, France.

PMID: 1691524
DOI: 10.1016/0923-2516(90)90059-r

Abstract

Replacing nick-translated DNA probes by in vitro transcribed complementary RNA (cRNA) probes considerably increased the sensitivity of dot-blot detection tests of potato spindle tuber viroid and chrysanthemum stunt viroid. As compared to the limit of detection of 5-10 pg of viroid obtained with 32P-labelled DNA probes, cRNA probes allow the detection of less than 1 pg of pure viroid. When labelled with biotin by incorporation of biotin-labelled ribonucleotides, the cRNA probes have a limit of detection of approximately 5 pg of purified viroid.

MeSH terms

Biotin
Molecular Probe Techniques
Phosphorus Radioisotopes
Plant Diseases
RNA / genetics
RNA Probes*
RNA, Complementary
RNA, Viral / genetics
Viroids / genetics
Viroids / isolation & purification*

Substances

Phosphorus Radioisotopes
RNA Probes
RNA, Complementary
RNA, Viral
RNA
Biotin