Abstract
The evolutionarily conserved actin-related protein (Arp2/3) complex is a key component of actin filament networks that is dynamically regulated by nucleation-promoting and inhibitory factors. Although much is known about actin assembly, the physiologic functions of inhibitory proteins are unclear. We generated coronin 1-/- mice and found that coronin 1 exerted an inhibitory effect on cellular steady-state F-actin formation via an Arp2/3-dependent mechanism. Whereas coronin 1 was required for chemokine-mediated migration, it was dispensable for T cell antigen receptor functions in T cells. Moreover, actin dynamics, through a mitochondrial pathway, was linked to lymphocyte homeostasis.
MeSH terms
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Actin-Related Protein 2-3 Complex / metabolism
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Actins / metabolism*
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Adoptive Transfer
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Animals
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Apoptosis
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / physiology
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Cell Polarity
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Cell Survival
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Chemotaxis, Leukocyte*
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Gene Targeting
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Homeostasis
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Intracellular Membranes / physiology
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Lymph Nodes / immunology
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Membrane Potentials
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Mice
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Mice, Inbred C57BL
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Microfilament Proteins / genetics
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Microfilament Proteins / physiology*
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Mitochondria / physiology*
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Receptors, Antigen, T-Cell / immunology
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / physiology
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology
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T-Lymphocytes / physiology*
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Talin / metabolism
Substances
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Actin-Related Protein 2-3 Complex
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Actins
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Microfilament Proteins
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Receptors, Antigen, T-Cell
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Talin
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coronin proteins