Abstract
We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.
MeSH terms
-
Animals
-
Endoplasmic Reticulum / ultrastructure*
-
Green Fluorescent Proteins / genetics*
-
Humans
-
Imaging, Three-Dimensional
-
Microscopy, Confocal
-
Microscopy, Fluorescence / instrumentation*
-
Microscopy, Fluorescence / nursing*
-
Microtubules / ultrastructure*
-
Nanotechnology
-
Nanovirus*
-
Protein Transport
-
Viral Proteins / genetics
-
Viral Proteins / metabolism
Substances
-
Viral Proteins
-
enhanced green fluorescent protein
-
Green Fluorescent Proteins