Nanoscale resolution in GFP-based microscopy

Nat Methods. 2006 Sep;3(9):721-3. doi: 10.1038/nmeth922.

Abstract

We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The approximately 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.

Publication types

  • Review

MeSH terms

  • Animals
  • Endoplasmic Reticulum / ultrastructure*
  • Green Fluorescent Proteins / genetics*
  • Humans
  • Imaging, Three-Dimensional
  • Microscopy, Confocal
  • Microscopy, Fluorescence / instrumentation*
  • Microscopy, Fluorescence / nursing*
  • Microtubules / ultrastructure*
  • Nanotechnology
  • Nanovirus*
  • Protein Transport
  • Viral Proteins / genetics
  • Viral Proteins / metabolism

Substances

  • Viral Proteins
  • enhanced green fluorescent protein
  • Green Fluorescent Proteins