Bioenergy beads: a tool for regeneration of ATP/NTP in biocatalytic synthesis

Artif Cells Blood Substit Immobil Biotechnol. 2006;34(5):515-21. doi: 10.1080/10731190600862886.

Abstract

Active inclusion bodies of recombinant polyphosphate kinase were obtained by simple washing of Escherichia coli cells with nonionic detergent and then they were immobilized in agar/TiO2 beads. Bioenergy beads obtained are charged by polyphosphate to act as rechargeable supply of adenosine/nucleoside triphosphates (ATP/NTP), a practical tool for synthesis of artificial receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Catalysis
  • Enzymes, Immobilized / biosynthesis
  • Enzymes, Immobilized / isolation & purification*
  • Escherichia coli / metabolism*
  • Escherichia coli Proteins / biosynthesis
  • Escherichia coli Proteins / isolation & purification*
  • Inclusion Bodies / metabolism*
  • Microspheres
  • Phosphotransferases (Alcohol Group Acceptor) / biosynthesis
  • Phosphotransferases (Alcohol Group Acceptor) / isolation & purification*

Substances

  • Enzymes, Immobilized
  • Escherichia coli Proteins
  • Adenosine Triphosphate
  • Phosphotransferases (Alcohol Group Acceptor)
  • polyphosphate kinase, E coli