Abstract
Active inclusion bodies of recombinant polyphosphate kinase were obtained by simple washing of Escherichia coli cells with nonionic detergent and then they were immobilized in agar/TiO2 beads. Bioenergy beads obtained are charged by polyphosphate to act as rechargeable supply of adenosine/nucleoside triphosphates (ATP/NTP), a practical tool for synthesis of artificial receptors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism*
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Catalysis
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Enzymes, Immobilized / biosynthesis
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Enzymes, Immobilized / isolation & purification*
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Escherichia coli / metabolism*
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Escherichia coli Proteins / biosynthesis
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Escherichia coli Proteins / isolation & purification*
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Inclusion Bodies / metabolism*
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Microspheres
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Phosphotransferases (Alcohol Group Acceptor) / biosynthesis
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Phosphotransferases (Alcohol Group Acceptor) / isolation & purification*
Substances
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Enzymes, Immobilized
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Escherichia coli Proteins
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Adenosine Triphosphate
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Phosphotransferases (Alcohol Group Acceptor)
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polyphosphate kinase, E coli