Toward intrinsically fluorescent proteomimetics: fluorescent probe response to alpha helix structure of poly-gamma-benzyl-L-glutamate

Kenji Hutt; Randy Hernandez; Michael D Heagy

doi:10.1016/j.bmcl.2006.07.050

Toward intrinsically fluorescent proteomimetics: fluorescent probe response to alpha helix structure of poly-gamma-benzyl-L-glutamate

Bioorg Med Chem Lett. 2006 Oct 15;16(20):5436-8. doi: 10.1016/j.bmcl.2006.07.050. Epub 2006 Aug 4.

Authors

Kenji Hutt¹, Randy Hernandez, Michael D Heagy

Affiliation

¹ New Mexico Institute of Mining and Technology, Chemistry, 801 Leroy Avenue, Socorro, NM 87801, USA.

PMID: 16890429
DOI: 10.1016/j.bmcl.2006.07.050

Abstract

A fluorescent probe (1), developed for recognition of alpha helical secondary structure, shows a large fluorescence change upon titration with the synthetic protein PBLG. Compared to fluorophores of similar size and shape, 1 displayed the smallest dissociation constant (K(D)=80microM) when titrated with PBLG. These preliminary studies are directed toward developing small molecule proteomimetics that have intrinsic fluorescence and are specific for helical-protein binding-sites.

Publication types

Comparative Study
Research Support, N.I.H., Extramural

MeSH terms

Fluorescent Dyes / chemistry*
Microscopy, Fluorescence / methods
Molecular Mimicry*
Molecular Structure
Polyglutamic Acid / analogs & derivatives*
Polyglutamic Acid / chemistry
Protein Structure, Secondary
Sensitivity and Specificity

Substances

Fluorescent Dyes
poly-gamma-benzyl-L-glutamate
Polyglutamic Acid