The total protein and total bilirubin levels as well as the GOT, GPT, alkaline phosphatase, GGTP and beta-glucuronidase activity in the blood serum and faeces from 121 diarrhoeic, Friesian-Holstein calves, aged from 1 to 10 days were investigated. As a control the results obtained in the previous investigations were used. Simultaneously the microbiological and histopathological examinations were performed. The changes of certain parameters in the blood serum, in the first 10 days of life, were parallel in both healthy and sick animals. The increase in alkaline phosphatase, GOT and GPT activity in calves with diarrhoea was observed. These changes may suggest the liver function damage during the neonatal calf diarrhoea. The decrease in GGTP activity, in the light of relationship between this enzyme and alkaline phosphatase activity, indicate the disturbances in intestinal absorption of colostral fraction of GGTP. The activity of beta-glucuronidase may be the sensitive index of liver function and indicate the range and the intensity of necrotic and/or dystrophic lesions. The activity of GOT and GPT in the faeces may be used as an indicator of liver function in the calves with diarrhoea while the additional investigations to explain the relationship between alkaline phosphatase, GGTP and beta-glucuronidase activity in faeces and intestinal lesions are needed.