Anticoagulant property of a semi-synthesized sodium beta-1,4-glucan sulfate

Yao Xue Xue Bao. 2006 Apr;41(4):323-7.

Abstract

Aim: To investigate the anticoagulant efficacy and mechanism of a semi-synthesized sodium beta-1,4-glucan sulfate (Na-MCS).

Methods: Anticoagulant activity was evaluated by means of coagulation assays in comparison with heparin. The anticoagulant mechanism of Na-MCS was disclosed by inhibitory analysis of the activities of coagulation factors using chromogenic substrates.

Results: 0.6 microg x mL(-1) Na-MCS could significantly prolong APTT and TT, but has less effect on PT at an even higher concentration. The dosage of Na-MCS required to double APTT of normal human plasma was 0.7 microg x mL(-1), lower than that of heparin with the activity of 150 u x mg(-1).

Conclusion: Na-MCS represented a potent anticoagulation activity in vitro, which matched the efficacy of heparin in a certain range of concentrations. Na-MCS exhibited anticoagulant activity due to inhibition of the coagulation factors IIa and Xa by the mediation of anti-thrombin AT-III.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anticoagulants / administration & dosage
  • Anticoagulants / pharmacology*
  • Antithrombin III / pharmacology
  • Blood Coagulation / drug effects*
  • Dose-Response Relationship, Drug
  • Factor Xa / metabolism
  • Glucans / administration & dosage
  • Glucans / pharmacology*
  • Heparin / pharmacology
  • Humans
  • Partial Thromboplastin Time
  • Prothrombin / metabolism
  • Prothrombin Time
  • Thrombin Time

Substances

  • Anticoagulants
  • Glucans
  • Antithrombin III
  • Prothrombin
  • Factor IIa
  • Heparin
  • 1,4-glucan
  • Factor Xa