Using five different series of recombinant inbred (RI) strains, we have mapped the gene encoding nebulin, a muscle-specific protein. The strain distribution pattern of Neb-specific RFLPs segregating in the RI strains showed significant concordance with those of Pmv-7 and Hc, two loci previously located to proximal mouse Chromosome 2. The gene order, intergene distances, and confidence intervals were determined according to standard maximum likelihood estimates as: centromere-Hc-5.4 cM (1.4 cM-15.4 cM; 95% confidence interval)-Neb-3.6 cM (1.5-6.7 cM)-Pmv-7.