Separation and determination of perfluorinated carboxylic acids using capillary zone electrophoresis with indirect photometric detection

J Chromatogr A. 2006 Sep 22;1128(1-2):290-7. doi: 10.1016/j.chroma.2006.06.049. Epub 2006 Jul 11.

Abstract

Perfluorinated carboxylic acids (PFCAs) belong to anthropogenic fluoroorganic compounds that have been detected in the natural environment and living organisms including humans. A capillary zone electrophoretic method with indirect UV detection using 2,4-dinitrobenzoic acid (2,4-DNBA) as a chromophore probe has been developed for analysis of PFCAs (C6-C12) in water. Optimal analyte resolution and detection sensitivity was obtained with 50 mM Tris solution of pH 9.0 and 50% methanol as a background electrolyte (BGE). The baseline separation of C6-C12 PFCAs was obtained within 20 min with detection limits in the range from 0.6 to 2.4 ppm.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carboxylic Acids / analysis
  • Carboxylic Acids / chemistry
  • Carboxylic Acids / isolation & purification*
  • Electrophoresis, Capillary / methods*
  • Fluorocarbons / analysis
  • Fluorocarbons / chemistry
  • Fluorocarbons / isolation & purification*
  • Hydrogen-Ion Concentration
  • Spectrophotometry, Ultraviolet / methods
  • Time Factors
  • Water / chemistry

Substances

  • Carboxylic Acids
  • Fluorocarbons
  • Water