Abstract
To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect <3 PFU of infectious poxvirus particles in <5 hours. This approach involved virus culture combined with real-time reverse transcription-polymerase chain reaction detection of 2 viral genes expressed immediately after infection.
MeSH terms
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Cell Line, Tumor
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DNA, Viral / isolation & purification
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Gene Expression Regulation, Viral
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Humans
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Polymerase Chain Reaction / methods
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RNA, Messenger / isolation & purification*
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RNA, Viral / isolation & purification*
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Sensitivity and Specificity
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Time Factors
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Vaccinia virus / isolation & purification*
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Virus Replication
Substances
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DNA, Viral
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RNA, Messenger
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RNA, Viral