[Application of fluorescence in-situ hybridization and reverse transcription-polymerase chain reaction in molecular diagnosis of Ewing's sarcoma and primitive neuroectodermal tumor]

Zhonghua Bing Li Xue Za Zhi. 2006 Jun;35(6):328-32.
[Article in Chinese]

Abstract

Objective: To detect tumor specific chromosome translocations and associated fusion transcripts in paraffin-embedded tissue by interphase fluorescence in-situ hybridization (FISH) and reverse transcription polymerase chain reaction (RT-PCR), respectively, and to evaluate their diagnostic values for Ewing's sarcoma/primitive neuroectodermal tumor (ES/PNET).

Methods: Nuclei of the tumor cells and total RNA were extracted from 10 cases of ES/PNET. Interphase FISH was utilized to analyze the EWS gene translocation with a dual color, break apart probe (Vysis company). RT-PCR was used to detect t (11; 22) (q24; q12) and t (21; 22) (q22; q12) fusion transcripts.

Results: Among 10 cases of ES/PNET, the EWS-FLI1 fusion transcript was detected in 8 by RT-PCR. EWS-ERG fusion transcript was not detected in any of the cases. EWS gene translocation was found in 9 of 10 cases by FISH.

Conclusions: Interphase FISH and RT-PCR can be reliably applied to paraffin-embedded tissues for molecular diagnosis of ES/PNET. Between the two approaches, interphase FISH provides a more sensitive and stable result.

Publication types

  • English Abstract

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Neuroectodermal Tumors, Primitive, Peripheral / diagnosis*
  • Neuroectodermal Tumors, Primitive, Peripheral / genetics
  • Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sarcoma, Ewing / diagnosis*
  • Sarcoma, Ewing / genetics