The neuroprotective effect of heme oxygenase (HO) on oxidative stress in HO-1 siRNA-transfected HT22 cells

Asuka Kaizaki; Sachiko Tanaka; Kumiko Ishige; Satoshi Numazawa; Takemi Yoshida

doi:10.1016/j.brainres.2006.06.011

The neuroprotective effect of heme oxygenase (HO) on oxidative stress in HO-1 siRNA-transfected HT22 cells

Brain Res. 2006 Sep 7;1108(1):39-44. doi: 10.1016/j.brainres.2006.06.011. Epub 2006 Jul 10.

Authors

Asuka Kaizaki¹, Sachiko Tanaka, Kumiko Ishige, Satoshi Numazawa, Takemi Yoshida

Affiliation

¹ Department of Biochemical Toxicology, School of Pharmaceutical Sciences, Showa University, 1-5-8 Hatanodai, Shinagawa, Tokyo 142-8555, Japan.

PMID: 16828716
DOI: 10.1016/j.brainres.2006.06.011

Abstract

To investigate the role of heme oxygenase (HO) isozymes, we used siRNA technology to suppress HO-1 expression. HO-1 siRNA-transfected HT22 cells were vulnerable to hydrogen peroxide- and 4-hydroxynonenal-induced cytotoxicity. Biliverdin and bilirubin, degradative products of heme catalyzed by HO, protected HT22 cells from the insult of these oxidative stressors. These results suggest that inducible HO-1 plays a protective role against oxidative stress in HT22 cells.

MeSH terms

Aldehydes / toxicity
Animals
Brain / enzymology*
Brain / physiopathology
Cell Line, Transformed
Down-Regulation / genetics
Gene Silencing / physiology
Heme Oxygenase-1 / genetics*
Hydrogen Peroxide / toxicity
Mice
Neurodegenerative Diseases / enzymology
Neurodegenerative Diseases / genetics
Neurodegenerative Diseases / physiopathology
Neuroprotective Agents / metabolism
Oxidative Stress / drug effects
Oxidative Stress / genetics*
RNA Interference

Substances

Aldehydes
Neuroprotective Agents
Hydrogen Peroxide
HMOX1 protein, human
Heme Oxygenase-1
4-hydroxy-2-nonenal