Soil organic carbon (SOC) has beneficial effects on soil quality and productivity. Cropping systems that maintain and/or improve levels of SOC may lead to sustainable crop production. This study evaluated the effects of long-term cropping systems on C sequestration. Soil samples were taken at 0- to 10-, 10- to 20-, 20- to 30-, and 30- to 40-cm soil depth profiles from grass pasture (GP), conventional tillage (CT) winter wheat (Triticum aestivum L.)-fallow (CTWF), and fertilized and unfertilized plots of continuous winter wheat (WW), spring wheat (SW), and spring barley (Hordeum vulgare L.) (SB) monocultures under CT and no-till (NT). The samples were analyzed for soil organic matter (SOM) and SOC was derived. Ages of experiments ranged from 6 to 73 yr. Compared to 1931 SOC levels (initial year), CTWF reduced SOC by 9 to 12 Mg ha(-1) in the 0- to 30-cm zone. Grass pasture increased SOC by 6 Mg ha(-1) in the 0- to 10-cm zone but decreased SOC by 3 Mg ha(-1) in the 20- to 30-cm zone. Continuous CT monocultures depleted SOC in the top 0- to 10-cm zone and the bottom 20- to 40-cm zone but maintained SOC levels close to 1931 SOC levels in the 10- to 20-cm layer. Continuous NT monocultures accumulated more SOC in the 0- to 10-cm zone than in deeper zones. Total SOC (0- to 40-cm zone) was highest under GP and continuous cropping and lowest under CTWF. Fertilizer increased total SOC only under CTWW and CTSB by 13 and 7 Mg ha(-1) in 13 yr, respectively. Practicing NT for only 6 yr had started to reverse the effect of 73 yr of CTWF. Compared to CTWF, NTWW and NTSW sequestered C at rates of 2.6 and 1.7 Mg ha(-1) yr(-1), respectively, in the 0- to 40-cm zone. This study showed that the potential to sequester C can be enhanced by increasing cropping frequency and eliminating tillage.