Advances in immunoproteomics for serological characterization of microbial antigens

Nandini Falisse-Poirrier; Virginie Ruelle; Benaissa ElMoualij; Danièle Zorzi; Olivier Pierard; Ernst Heinen; Edwin De Pauw; Willy Zorzi

doi:10.1016/j.mimet.2006.05.002

Advances in immunoproteomics for serological characterization of microbial antigens

J Microbiol Methods. 2006 Dec;67(3):593-6. doi: 10.1016/j.mimet.2006.05.002. Epub 2006 Jul 5.

Authors

Nandini Falisse-Poirrier¹, Virginie Ruelle, Benaissa ElMoualij, Danièle Zorzi, Olivier Pierard, Ernst Heinen, Edwin De Pauw, Willy Zorzi

Affiliation

¹ Center of Research on Prion Proteins, Institute of Pharmacy-CHU, B36, Avenue de l'Hôpital 1, B-4000 University of Liège, Belgium.

PMID: 16822569
DOI: 10.1016/j.mimet.2006.05.002

Abstract

We propose a multi-dimensional strategy, associating immunodetection to a protein fractionating two-dimensional liquid chromatography tool, for serological characterization of microbial antigens. The originality of such immunoproteomic approaches resides in their application in large-scale studies for rapid serotyping of micro-organisms, evaluation of immunomes and could be proposed in the development and monitoring of vaccines.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Bacterial / analysis*
Antigens, Bacterial / immunology*
Bacillus subtilis / immunology*
Bacterial Proteins / analysis*
Bacterial Proteins / immunology*
Blotting, Western
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Immunologic Techniques
Microscopy / methods
Proteome / analysis
Proteomics*
Serotyping
Spectrum Analysis
Tandem Mass Spectrometry

Substances

Antigens, Bacterial
Bacterial Proteins
Proteome