Growth factor stimulation of proliferating cell nuclear antigen (PCNA) in human breast epithelium in organ culture

Cell Biol Int Rep. 1991 Jul;15(7):561-70. doi: 10.1016/0309-1651(91)90003-2.

Abstract

Normal human breast explants were maintained in serum-free culture for 7 days in the presence of either insulin hydrocortisone and cholera toxin (I/H/CT), epidermal growth factor (EGF) or transforming growth factor-alpha (TGF-alpha). Explants were labelled with [3H] thymidine, fixed in methacarn and processed for autoradiography. Parallel sections were immunolabelled with anti-PCNA antibody and analysed with a CAS 200 image analyser. Thymidine labelling index (TLI) and PCNA expression produced similar results with both indices increased in response to I/H/CT, EGF and TGF-alpha. In sections double labelled for PCNA and autoradiography the majority of labelled cells were positive for both markers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantigens / metabolism
  • Breast / drug effects*
  • Breast / immunology
  • Breast / metabolism
  • Cell Division / drug effects
  • Cholera Toxin / pharmacology
  • Epidermal Growth Factor / pharmacology
  • Epithelial Cells
  • Epithelium / immunology
  • Epithelium / metabolism
  • Female
  • Growth Substances / pharmacology*
  • Humans
  • Hydrocortisone / pharmacology
  • Insulin / pharmacology
  • Nuclear Proteins / immunology*
  • Nuclear Proteins / metabolism
  • Organ Culture Techniques
  • Proliferating Cell Nuclear Antigen
  • Thymidine / metabolism
  • Transforming Growth Factor alpha / pharmacology

Substances

  • Autoantigens
  • Growth Substances
  • Insulin
  • Nuclear Proteins
  • Proliferating Cell Nuclear Antigen
  • Transforming Growth Factor alpha
  • Epidermal Growth Factor
  • Cholera Toxin
  • Thymidine
  • Hydrocortisone