A 57-year-old man presented with a liver mass that had been detected on physical examination. The abdominal computed tomography scan revealed a 5-cm single nodular hepatoma located in segments 6 and 7. A total laparoscopic right posterior sectionectomy was performed for this lesion. The anatomical demarcation of the posterior section was possible with selective control of a Glissonian pedicle to that section. The patient was discharged on postoperative day 13 without complications. The postoperative pathology confirmed a hepatocellular carcinoma with a 1-cm free resection margin. The patient had no evidence of recurrence at 12-month follow-up. To our knowledge, this is the first reported case of total laparoscopic right posterior sectionectomy in segments 6 and 7.