In situ hybridization of whole-mount embryos

Methods Mol Biol. 2006:326:103-13. doi: 10.1385/1-59745-007-3:103.

Abstract

The ability to visualize the expression of a gene in both time and space is an essential tool of developmental biology. Here, we detail a robust method for in situ hybridization of RNA probes to whole pieces of fixed tissue. This method has been optimized for reliable and sensitive visualization of the spatial patterns of gene expression in mouse embryo tissue.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Embryo, Mammalian / cytology*
  • Female
  • Gene Expression
  • In Situ Hybridization / methods*
  • Mice
  • Nucleic Acid Hybridization / methods
  • Pregnancy
  • RNA Probes / chemical synthesis
  • Transcription, Genetic

Substances

  • RNA Probes