High-resolution NMR spectroscopy of a GPCR in aligned bicelles

J Am Chem Soc. 2006 Jun 14;128(23):7402-3. doi: 10.1021/ja0606632.

Abstract

Solid-state NMR spectra with single-site resolution of CXCR1, a G protein-coupled receptor (GPCR), were obtained in magnetically aligned phospholipid bicelles. These results demonstrate that GPCRs in phospholipid bilayers are suitable samples for structure determination by solid-state NMR. The spectra also enable studies of drug-receptor interactions.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / metabolism
  • Lipid Bilayers / chemistry*
  • Lipid Bilayers / metabolism
  • Magnetic Resonance Spectroscopy / methods
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Receptors, G-Protein-Coupled / chemistry*
  • Receptors, G-Protein-Coupled / metabolism
  • Receptors, Interleukin-8A / analysis
  • Receptors, Interleukin-8A / chemistry*
  • Receptors, Interleukin-8A / metabolism

Substances

  • Lipid Bilayers
  • Receptors, G-Protein-Coupled
  • Receptors, Interleukin-8A
  • GTP-Binding Proteins