Abstract
Prions have been described in mammals and fungi. The [Het-s] infectious genetic element of the filamentous fungus Podospora anserina is the prion form of the HET-s protein. This protein is involved in the control of a cell death reaction termed heterokaryon incompatibility. The infectious form of HET-s corresponds to a self-perpetuating amyloid. The purpose of the present paper is to describe the techniques that can be used to analyse [Het-s] prion propagation in vivo and HET-s amyloid aggregation in vitro. In addition, we report several methods that can be used to infect Podospora with recombinant HET-s amyloid.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyloid / chemistry
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Amyloid / ultrastructure
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Circular Dichroism
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Gene Transfer Techniques
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Microscopy, Electron
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Podospora / genetics
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Podospora / metabolism*
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Prions / genetics
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Prions / metabolism*
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Prions / ultrastructure
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Spectrometry, Fluorescence
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Spores, Fungal / genetics
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Spores, Fungal / metabolism
Substances
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Amyloid
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Fungal Proteins
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HET-S protein, Podospora anserina
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Prions
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Recombinant Fusion Proteins
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Green Fluorescent Proteins