Afferent and efferent connections of the cerebellum of a salmonid, the rainbow trout (Oncorhynchus mykiss): a tract-tracing study

Abstract

The connections of the cerebellum of the rainbow trout were studied by experimental methods. The pretectal paracommissural nucleus has reciprocal connections with the cerebellum. Three additional pretectal nuclei project to both the corpus and valvula cerebelli, and seem to receive cerebellar afferents. A large number of cells of the lateral nucleus of the valvula project to wide regions of the cerebellum, including the valvula, the corpus, the granular eminences, and the caudal lobe, whereas the contralateral inferior olive and scattered reticular cells project only to the corpus and valvula cerebelli. Afferents to the corpus were also observed from the ventral tegmental nucleus, the "paraisthmic nucleus," the perilemniscal nucleus, the central gray, and the octavolateral area. Valvular afferents were also observed from the torus semicircularis and the midbrain tegmental areas. In most cases of cerebellar application, labeled fibers were seen in the thalamus, the pretectum, the torus longitudinalis and torus semicircularis, the nucleus of the medial longitudinal fascicle, and midbrain and rhombencephalic reticular areas. From the corpus cerebelli some fibers also project to the posterior tubercle and the hypothalamus. Moreover, the granular eminences project to the cerebellar crest. DiI application to most of the areas showing labeled fibers after cerebellar tracer application led to the labeling of characteristic eurydendroid cells, mainly in the valvula cerebelli and the caudal lobe. A few putative eurydendroid cells were labeled from the octavolateralis regions. These results in a teleost with a generalized brain indicate several differences with respect to the cerebellar connections reported in other teleost fishes that have specialized brains.