Double-stranded RNA (dsRNA) gene interference is an efficient method to silence gene expression in a sequence specific manner. Here we show, that dsRNA targeting the allatostatin (AS)-A type (FGL/I/V-amide) gene of Gryllus bimaculatus (Ensifera, Gryllidae) and Spodoptera frugiperda (Lepidoptera, Noctuidae) injected into freshly moulted larvae or adult crickets and moths produced a rapid and long-lasting reduction in the mRNA levels in various tissues. The effect lasted up to 7 days. Following dsRNA injection, the juvenile hormone (JH) titers in the hemolymph were clearly raised in both species. AS-dsRNA injection induced a reduced body weight in larval and adult crickets and the imaginal moult was incomplete. Silencing allatostatin type-A expression also reduced the egg and testes development in crickets, and the oviposition rate was drastically diminished in both species.