Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability

Benjamin Erable; Isabelle Goubet; Sylvain Lamare; Marie-Dominique Legoy; Thierry Maugard

doi:10.1016/j.chemosphere.2006.04.007

Bioremediation of halogenated compounds: comparison of dehalogenating bacteria and improvement of catalyst stability

Chemosphere. 2006 Nov;65(7):1146-52. doi: 10.1016/j.chemosphere.2006.04.007. Epub 2006 May 24.

Authors

Benjamin Erable¹, Isabelle Goubet, Sylvain Lamare, Marie-Dominique Legoy, Thierry Maugard

Affiliation

¹ Laboratoire de Biotechnologies et de Chimie Bio-organique CNRS FRE 2766, Bâtiment Marie Curie, Université de La Rochelle, Avenue Michel Crépeau 17042 La Rochelle cedex 1, France.

PMID: 16723151
DOI: 10.1016/j.chemosphere.2006.04.007

Abstract

Five bacterial strains were compared for halogenated compounds conversion in aqueous media. Depending on the strain, the optimal temperature for dehalogenase activity of resting cells varied from 30 to 45 degrees C, while optimal pH raised from 8.4 to 9.0. The most effective dehalogenase activity for 1-chlorobutane conversion was detected with Rhodococcus erythropolis NCIMB13064 and Escherichia coli BL21 (DE3) (DhaA). The presence of 2-chlorobutane or propanal in the aqueous media could inhibit the 1-chlorobutane transformation.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
Bioreactors
Butanes / metabolism
Catalysis
Environmental Pollutants / metabolism*
Escherichia coli / enzymology
Escherichia coli / genetics
Hydrocarbons, Halogenated / metabolism*
Hydrogen-Ion Concentration
Hydrolases / genetics
Hydrolases / metabolism*
Rhodococcus / enzymology
Sphingomonas / enzymology
Temperature
Xanthobacter / enzymology

Substances

Butanes
Environmental Pollutants
Hydrocarbons, Halogenated
Hydrolases
haloalkane dehalogenase
butyl chloride