Determination of the big head carp myofibrillar (Aristichthys nobilis) adenosine triphosphatase activity by ion chromatography

Rui-chang Gao; Chang-hu Xue; Li Yuan; Zhao-jie Li; Yong Xue; Fengxia Cui; Yan Sun

doi:10.1016/j.chroma.2006.04.066

Determination of the big head carp myofibrillar (Aristichthys nobilis) adenosine triphosphatase activity by ion chromatography

J Chromatogr A. 2006 Jun 23;1118(2):278-80. doi: 10.1016/j.chroma.2006.04.066. Epub 2006 May 15.

Authors

Rui-chang Gao¹, Chang-hu Xue, Li Yuan, Zhao-jie Li, Yong Xue, Fengxia Cui, Yan Sun

Affiliation

¹ College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, China.

PMID: 16701679
DOI: 10.1016/j.chroma.2006.04.066

Abstract

A method for the determination of adenosine triphosphatase (ATPase) activity of myofibrils of big head carp by using ion chromatography was introduced. Adenosine triphosphate (ATP), adenosine diphosphate (ADP) and orthophosphate (Pi) were separated completely. Recoveries for ATP, ADP and Pi were 98+/-5%, 97+/-4% and 98+/-5%, respectively. Pi liberated from ATP during reaction was monitored by ion chromatography using the suggested method. This method was applicable to the determination of myofibrils ATPase activity for quick quality evaluation of surimi.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / metabolism*
Adenosine Triphosphate / metabolism
Animals
Calibration
Carps
Chromatography, Liquid / methods*
Hydrolysis
Reproducibility of Results

Substances

Adenosine Triphosphate
Adenosine Triphosphatases