Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin

Constança Pomba; Nuno Mendonça; Marta Costa; Deolinda Louro; Bruno Baptista; Mónica Ferreira; José Duarte Correia; Manuela Caniça

doi:10.1016/j.diagmicrobio.2006.03.005

Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin

Diagn Microbiol Infect Dis. 2006 Sep;56(1):103-6. doi: 10.1016/j.diagmicrobio.2006.03.005. Epub 2006 May 5.

Authors

Constança Pomba¹, Nuno Mendonça, Marta Costa, Deolinda Louro, Bruno Baptista, Mónica Ferreira, José Duarte Correia, Manuela Caniça

Affiliation

¹ CIISA, Faculty of Veterinary Medicine, Universidade Técnica de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal. cpomba@fmv.utl.pt

PMID: 16678992
DOI: 10.1016/j.diagmicrobio.2006.03.005

Abstract

We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cat Diseases / microbiology
Cats
Dog Diseases / microbiology
Dogs
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / genetics
Escherichia coli Infections / veterinary*
Genes, Bacterial
Genotype
Polymerase Chain Reaction / methods*
Swine
Swine Diseases / microbiology
Urinary Tract Infections / microbiology
Urinary Tract Infections / veterinary*
beta-Lactam Resistance / genetics*