Abstract
The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Biomarkers
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Bone Morphogenetic Protein Receptors / genetics
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Bone Morphogenetic Protein Receptors / metabolism
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Bone Morphogenetic Proteins / metabolism*
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Deoxyribonucleases, Type II Site-Specific / genetics
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Gene Expression Regulation, Developmental
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Hematopoiesis*
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Kidney / blood supply
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Kidney / embryology
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Kidney / metabolism
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Mesoderm / metabolism*
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PAX2 Transcription Factor / metabolism
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Saccharomyces cerevisiae Proteins
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Signal Transduction*
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Somites / metabolism*
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Stem Cells / metabolism
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Time Factors
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Zebrafish / embryology
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Zebrafish / genetics
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Zebrafish / metabolism
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Zebrafish Proteins / metabolism
Substances
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Biomarkers
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Bone Morphogenetic Proteins
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PAX2 Transcription Factor
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Saccharomyces cerevisiae Proteins
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Zebrafish Proteins
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pax2a protein, zebrafish
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Bone Morphogenetic Protein Receptors
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SCEI protein, S cerevisiae
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Deoxyribonucleases, Type II Site-Specific