Infertility has been considered a global public health problem in many countries worldwide. Our objective was to restore endocrine function and fertility in tubal-oophorectomized rabbits using an orthotopic tubal-ovarian vascularized autotransplant model as the anatomical-functional unit while employing a microvascular surgical technique. Twenty New Zealand white (NZW) sexually mature female rabbits and four male NZW rabbits of proven fertility were divided into two study groups. In group I (n = 10), a left salpingo-oophorectomy was performed. Group II (n = 10) was subjected to a bilateral salpingo-oophorectomy, plus a right orthotopic tubal-ovarian autotransplant. Our testing variables were vascular and tubal-anastomoses permeability, estradiol (E2) and progesterone (P4) serum levels, pregnancy, number of offspring, histopathological study of the uteri, fallopian tubes, and ovaries. One hundred percent immediate permeability of the tubal anastomoses was achieved, while late permeability was found to be 64%. Immediate permeability of vascular anastomoses was 90%, and late permeability was recorded at 80%. E2 serum levels in both groups at different times showed no statistically significant differences. In the case of P4, a small difference was found during pregnancy, especially greater in the control group (P < .05). In the autotransplanted group, four rabbits became pregnant (44%). Endocrine function and fertility were restored in the rabbits with the tubal-ovarian transplant as the anatomical-functional unit. The use of isotransplants and allotransplants should be considered a therapeutic alternative in the infertile woman with irreparable bilateral tubal damage, ovarian dysgenesis, surgical absence of ovaries and fallopian tubes, or when the conventional IVF/TE in these cases has been unsuccessful.