During recent years, a great deal of research has focused on the identification of food allergens, their characterization at the molecular level, and the investigation of immunological mechanisms responsible for food hypersensitivity. Allergen-reactive T-helper 2 cells play a central role in the pathophysiology of allergic diseases. After activation with allergens, this subset of CD4(+) T-lymphocytes produces a spectrum of cytokines that induce high serum levels of immunoglobulin E and eosinophilia, which, in turn, mediate the clinical symptoms. Furthermore, T-helper 2 cells are present in target organs during the late response to allergen exposure. The analysis of the allergic T-cell response, including cytokine production and the identification of T-cell epitopes, provides important information on the characteristics of food allergens. Therefore, in vitro cultures of allergen-specific T-cells derived from peripheral blood or different target organs have been of indispensable help. Results obtained from such studies may not only contribute to the understanding of cellular mechanisms underlying different types of food allergy but also to the development of novel strategies for immunotherapy of food allergy.