The gene frequency of 5 characters was reported in Buyi, Miao, Shui, Maonan, Dong, and Han nationalities in southern Guizhou, and analyzed for statistical significance among these nationalities. The results showed that: (1) For Hair forms and Nasal profile, the frequency of the dominant gene was lower than that of theirs recessive gene, but the opposite was true for Nostril forms. (2) Among different nationalities, the difference of gene frequency of Mongoloid fold, Nasal profile, Hair forms and Eye fold of the upper eyelid was quite significant, which was followed by that of Nostril forms.