ESAT-6 peptide recognition by bovine CD8+ lymphocytes of naturally infected cows in herds from southern Italy

Fabrizio Vitale; Stefano Reale; Enrico Petrotta; Santo Caracappa; Annalisa Barera; Marco Pio La Manna; Pasquale Macaluso; Nadia Caccamo; Francesco Dieli; Hans Martin Vordermeier; Guido Sireci; Alfredo Salerno

doi:10.1128/CVI.13.4.530-533.2006

ESAT-6 peptide recognition by bovine CD8+ lymphocytes of naturally infected cows in herds from southern Italy

Clin Vaccine Immunol. 2006 Apr;13(4):530-3. doi: 10.1128/CVI.13.4.530-533.2006.

Authors

Fabrizio Vitale¹, Stefano Reale, Enrico Petrotta, Santo Caracappa, Annalisa Barera, Marco Pio La Manna, Pasquale Macaluso, Nadia Caccamo, Francesco Dieli, Hans Martin Vordermeier, Guido Sireci, Alfredo Salerno

Affiliation

¹ Istituto Zooprofilattico Sperimentale della Sicilia, Via G. Marinuzzi 3, 90100 Palermo, Italia.

Abstract

The aim of this study was to define epitopes of Mycobacterium bovis from ESAT-6 (early secretory antigen of 6 kDa) recognized by CD8+ T lymphocytes from cows naturally infected with Mycobacterium bovis. We found that bovine CD8+ T cells recognized 10 out of 11 ESAT-6 peptides tested.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antigens, Bacterial / immunology*
Antigens, Bacterial / metabolism
Bacterial Proteins
CD8-Positive T-Lymphocytes / immunology*
CD8-Positive T-Lymphocytes / metabolism
CD8-Positive T-Lymphocytes / microbiology
Cattle
Cells, Cultured
Epitopes, T-Lymphocyte / immunology
Female
Interferon-gamma / metabolism
Lymphocyte Activation / immunology
Mice
Molecular Sequence Data
Mycobacterium bovis / immunology*
Sicily
Tuberculosis, Bovine / immunology*

Substances

Antigens, Bacterial
Bacterial Proteins
ESAT-6 protein, Mycobacterium tuberculosis
Epitopes, T-Lymphocyte
Interferon-gamma